Phosphorylation and Protein Synthetic Events in Xenopus laevis Oocytes Microinjected with pp60^v-src

Abstract

Microinjection of purified pp60^v-src, the transforming protein of Rous sarcoma virus, into Xenopus laevis oocytes accelerated the rate of progesterone- or insulin-induced meiotic maturation. This acceleration was abolished by incubating the oocytes with cycloheximide or puromycin during a 2-h interval between pp60^v-src microinjection and progesterone addition. In contrast, exposure to actinomycin D did not alter the acceleration of maturation by microinjected pp60^v-src. Associated with progesterone treatment and pp60^v-src microinjection were a number of qualitative changes in phosphoproteins; a few of these changes are common to both stimuli. These results indicate that the action of pp60^v-src in oocytes involves both phosphorylation and protein synthetic events that affect oocyte maturation.