Context-Dependent Gene Expression: cis-Acting Negative Effects of Specific Procaryotic Plasmid Sequences on Eucaryotic Genes

David O. PetersonDepartment of Biochemistry and Biophysics, Texas Agricultural Experiment Station, Texas A & M University, College Station, Texas77843

Katherine K. BeifussDepartment of Biochemistry and Biophysics, Texas Agricultural Experiment Station, Texas A & M University, College Station, Texas77843

Kimberlin L. MorleyDepartment of Biochemistry and Biophysics, Texas Agricultural Experiment Station, Texas A & M University, College Station, Texas77843

Abstract

A sequence element within pBR322 DNA mediates a cis-acting negative effect on expression from eucaryotic genes in transient expression assays. The negative element overlaps with sequences that inhibit DNA replication, but its effect is observed in the absence of detectable replication of transfected DNA.

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Context-Dependent Gene Expression: cis-Acting Negative Effects of Specific Procaryotic Plasmid Sequences on Eucaryotic Genes

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Context-Dependent Gene Expression: cis-Acting Negative Effects of Specific Procaryotic Plasmid Sequences on Eucaryotic Genes

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