Augmented Mitogenic Responsiveness to Epidermal Growth Factor in Murine Fibroblasts That Overexpress pp60^c-src

Abstract

C3H10T1/2 murine fibroblasts overexpressing chicken pp60^c-src showed a two- to fivefold enhanced incorporation of [³H]thymidine into DNA in response to epidermal growth factor (EGF) relative to that of the parent line. No difference in growth characteristics, number and affinity of EGF receptors, or hormone potency was attributable to c-src overexpression. These results suggest that pp60^c-src may interact with the mitogenic signal transduction pathway of EGF in some event distal to hormone binding.