Isolation of cDNA Clones for Mouse Cytoskeletal γ-Actin and Differential Expression of Cytoskeletal Actin mRNAs in Mouse Cells

Abstract

We described the structures of mouse cytoskeletal γ-actin cDNA clones and showed that there is strong conservation of the untranslated regions with human γ-actin cDNA. In addition, we found that the expression levels of β- and γ-actin mRNAs are differentially controlled in various mouse tissues and cell types but are coordinately increased in the cellular growing state. These results suggest that there are multiple regulatory mechanisms of cytoskeletal actin genes and are consistent with the argument that β- and γ-actins might have functional diversity in mammalian cells.