Notes: Transfection of a Human Gene That Corrects the Lec1 Glycosylation Defect: Evidence for Transfer of the Structural Gene for N-Acetylglucosaminyltransferase I

Abstract

Chinese hamster ovary (CHO) glycosylation mutants provide an approach to cloning mammalian glycosyl* transferases by transfection and gene rescue. In this paper, complementation of the lec1 CHO mutation by human DNA is described. Led transfectants expressed human N-acetylglucosaminyltransferase I (GlcNAc-TI) activity and possessed common human DNA fragments. Cloning of GlcNAc-TI should therefore be possible.

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Tumor Necrosis Factor Induces the Rapid Phosphorylation of the Mammalian Heat Shock Protein hsp28

Transfection of a Human Gene That Corrects the Lecl Glycosylation Defect: Evidence for Transfer of the Structural Gene for N-Acetylglucosaminyltransferase I