Expression of Protein Kinase C Genes during Ontogenic Development of the Central Nervous System

Abstract

We have analyzed the RNA expression of three protein kinase C (PKC) genes (α, β, and γ) in human and murine central nervous systems during embryonic-fetal, perinatal, and adult life. Analysis of human brain poly(A)⁺ RNA indicates that expression of PKC α and β genes can be detected as early as 6 weeks postconception, undergoes a gradual increase until 9 weeks postconception, and reaches its highest level in the adult stage, and that the PKC γ gene, although not expressed during embryonic and early fetal development, is abundantly expressed in the adult period. Similar developmental patterns were observed in human spinal cord and medulla oblongata. A detailed analysis of PKC gene expression during mammalian ontogeny was performed on poly(A)⁺ RNA from the brain cells of murine embryos at different stages of development and the brain cells of neonatal and adult mice. The ontogenetic patterns were similar to those observed for human brain. Furthermore, we observed that the expression of PKC γ is induced in the peri- and postnatal phases. These results suggest that expression of PKC α, β, and γ genes possibly mediates the development of central neuronal functions, and expression of PKC γ in particular may be involved in the development of peri- and postnatal functions.