Telomere Terminal Transferase Activity from Euplotes crassus Adds Large Numbers of TTTTGGGG Repeats onto Telomeric Primers

Abstract

A telomere terminal transferase activity was identified in developing macronuclear extracts from Euplotes crassus. The activity was essentially unregulated in vitro: up to 50 tandem repeats of the Euplotes telomeric repeat sequence TTTTGGGG were added onto synthetic telomeric oligonucleotide primers. Both the structure of the telomere substrate and its 3′-terminal sequence were recognized. The activity was destroyed by low concentrations of RNase A.