PEGylated Guanidinylated Polyallylamine as Gene-Delivery Carrier

Yi-Chen Chung Institute of Polymer Science and Engineering, College of Engineering, National Taiwan University, Taipei 106, Taiwan

Ming-Feng Wei Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 100, Taiwan

Fu-Hsiung Chang Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Taipei 100, Taiwan

Tai-Horng Young Institute of Polymer Science and Engineering, College of Engineering, National Taiwan University, Taipei 106, Taiwan; Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 100, Taiwan. [email protected]

Abstract

A novel cationic co-polymer was developed by grafting poly(ethylene glycol) (PEG) on guanidinylated polyallylamine (PAA) for gene delivery. Characterization of PEG-g-guanidinylated PAA/DNA complexes demonstrated that particle size increased and surface charge decreased with increasing the amount of PEG. The results of cytotoxicity assay proved that grafted PEG could effectively decrease the cytotoxicity of the complexes. In transfection efficiency assay, HeLa cells treated with PEG(2)-g-guanidinylated PAA (formed with 17.5 μmol guanidinylated PAA and 2 μmol PEG)/DNA (0.2 μg EGFP plasmid) complexes showed a very high level of EGFP expression. In conclusion, combination of guanidinylation and PEGylation could effectively decrease the cytotoxicity and significantly increase the transfection efficiency of PAA.

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PEGylated Guanidinylated Polyallylamine as Gene-Delivery Carrier

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PEGylated Guanidinylated Polyallylamine as Gene-Delivery Carrier

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