Study of cellular responses to polymeric biomaterials using the differential display method

Shinya Kato Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University

Akio Kishida Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University

Naohiro Hanyu Department of Clinical Laboratory Medicine, Faculty of Medicine, Kagoshima University, 8-35

Ikuro Maruyama Department of Clinical Laboratory Medicine, Faculty of Medicine, Kagoshima University, 8-35

Mitsuru Akashi Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, 1-21-40, Korimoto, Kagoshima 890-0065, Japan 2 Department of Clinical Laboratory Medicine, Faculty of Medicine, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima 890-0075, Japan

Abstract

In this study, we attempted to detect altered gene expressions in the cells that had adhered to various surfaces using the differential display method. Thioglycollate-elicited peritoneal exudate cells (PEC) and mouse fibroblast (L929) cells were cultured on the polymer films. After a predetermined time, the total RNA was isolated from cells and the differential mRNA expressions were evaluated by RT-PCR method. As a result, in the differential display of amplified cDNA from PEC, the different patterns of cDNA fragments among the samples were obtained. This indicates that there were many different mRNA expressions depending on the polymer surfaces. The use of differential method was proven to be useful for studying cell-polymer interaction.

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Study of cellular responses to polymeric biomaterials using the differential display method

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Study of cellular responses to polymeric biomaterials using the differential display method

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