BHK cell attachment and growth on EDA-plasma-modified poly(L-lactide/ε-caprolactone) biodegradable films

Abstract

In this study, attachment and growth of Baby Hamster Kidney (BHK) cells on ethylene diamine (EDA)-plasma-treated poly(L-lactide/ε-caprolactone) biodegradable copolymer films were investigated. The co-polymer (M_w: 58 000; M_n: 35 000 and PI 1.60) was synthesised by ring-opening polymerization of the respective dimers with using stannous octoate as the catalyst. The final ratio of L-lactide to ε-caprolactone obtained by ¹H-NMR was 87 :13. The co-polymer films were treated with the EDA-plasma in a glow-discharge apparatus. The BHK-30 cell line was cultured on plain and EDA-plasma-treated films and their pre-wetted forms (with ethanol and/or cell culture medium before use). Cell attachment and growth were followed. Alkaline phosphatase (ALP) activity and glucose uptake in cell culture medium were also investigated. There was no attachment in the first 12 h. Glow-discharge treatment increased significantly the attachment and growth. Pre-wetting with ethanol and cell culture medium was also increase significantly both the attachment and growth.

Keywords:

• BHK CELLS
• POLY L-LACTIDE EPSILON-CAPROLACTONE
• EDA-PLASMA TREATMENT
• PRE-WETTING
• CELL
• ATTACHMENT
• CELL GROWTH
• ALP ACTIVITY
• GLUCOSE UPTAKE.