In vitro thrombogenicity investigation of new water-dispersible polyurethane anionomers bearing carboxylate groups

Abstract

New segmented polyurethane (PU) anionomers based on hydroxytelechelic polybutadiene were synthesized via an aqueous dispersion process. Incorporation of carboxylic groups was achieved using thioacids of different length. Surface properties were investigated by mean of water absorption analysis and static contact-angle measurements using water, diiodomethane, formamide and ethylene glycol. Blood compatibility of the PUs was evaluated by in vitro adhesion assays using ¹¹¹In-radiolabeled platelet-rich plasma and [¹²⁵I]fibrinogen. Morphology of the adhered platelets was examined by scanning electron microscopy (SEM). Results were compared to two biomedicalgrade PUs, namely Pellethane^® and Tecoflex^®. Insertion of carboxylic groups increased surface hydrophilicity and limited water uptake (<8% for an ion content of 5% by weight). Surface energy of all synthesized PUs was between 40 and 45 mJ/m². Platelet adhesion and fibrinogen adsorption on the PU anionomer surfaces were affected as a function to the increase of graft length; thiopropionic was the most haemocompatible, followed by thiosuccinic and then thioglycolic acid. SEM analyses of all ionic PU samples exhibited low platelet adhesion to surfaces with no morphological modification. In conclusion, increased hydrophily, dynamic mobility and charge repulsion are synergistic key factors for enhanced haemocompatibility.

Keywords:

• POLYURETHANE ANIONOMERS
• IN VITRO HAEMOCOMPATIBILITY ASSAYS
• PLATELET ADHESION
• FIBRINOGEN ADSORPTION
• ELECTRON MICROSCOPY