Lysosomotropic detergents induce time- and dose-dependent apoptosis/necrosis in cultured cells

Abstract

Oxidative stress has been found to cause lysosomal rupture due to iron-catalyzed intralysosomal oxidative reactions.^1,2 Moderate rupture induces apoptosis, while necrosis follows a more complete relocation of this type.² We have suggested that lysosomal cysteine proteases may directly activate the caspase cascade and, together with other lysosomal hydrolases, induce mitochondrial release of cytochrome c with ensuing further activation of the cascade.