A comparison of the gene expression profiles of CRL-1807 colonocytes exposed to endogenous AAPH-generated peroxides and exogenous peroxides from heated oil

Eugene P. HalliganMolecular Toxicology GroupDepartment of Forensic Science and Drug Monitoring, School of Health and Life Sciences, King's College London, London, UK

Damon LowesAcademic Unit of Anaesthesia and Intensive CareUniversity of Aberdeen, Aberdeen, UK

Nalini MistryRadiation and Oxidative Stress GroupDepartment of Cancer Studies and Molecular Medicine, Leicester Royal Infirmary, University Hospitals of Leicester NHS Trust, Leicester, UK

Rosamund DoveMolecular Toxicology GroupDepartment of Forensic Science and Drug Monitoring, School of Health and Life Sciences, King's College London, London, UK

Marcus CookeRadiation and Oxidative Stress GroupDepartment of Cancer Studies and Molecular Medicine, Leicester Royal Infirmary, University Hospitals of Leicester NHS Trust, Leicester, UK

Mark EvansRadiation and Oxidative Stress GroupDepartment of Cancer Studies and Molecular Medicine, Leicester Royal Infirmary, University Hospitals of Leicester NHS Trust, Leicester, UK

Joseph LunecMolecular Toxicology GroupDepartment of Forensic Science and Drug Monitoring, School of Health and Life Sciences, King's College London, London, UK

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Abstract

Oxidation of PUFAs in the diet has the potential to be genotoxic and hence carcinogenic. Such carcinogenic processes originate within stem cells of the colon. These cells appear to be predisposed to the carcinogenic process. In colon cells (CRL-1807) exposed to chemical reactions simulating exogenous and endogenous peroxidation reactions, we have observed that undifferentiated cells could mount an effective recombinational repair/TCR response to an endogenous peroxidative DNA damage insult, but not to an external exogenous peroxidative insult as one would encounter from a dietary source. This may suggest that defects in such specific DNA repair may play a role in tumour development in undifferentiated colonocytes exposed to a diet-derived lipid peroxides.

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A comparison of the gene expression profiles of CRL-1807 colonocytes exposed to endogenous AAPH-generated peroxides and exogenous peroxides from heated oil

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A comparison of the gene expression profiles of CRL-1807 colonocytes exposed to endogenous AAPH-generated peroxides and exogenous peroxides from heated oil

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