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Abstract
We describe a simplified procedure for processing brain tissue for both light and electron microscopy. High resolution images displaying excellent tissue morphology and sharp contrast in structural components of brain cells were obtained. Sections were cut on a vibrating microtome, processed in the free-floating form, and mounted on Teflon coated slides. The desired area of interest was marked after light microscopic analysis and tracings or photographs were done. The minute piece of tissue was then extracted from the block, reembedded on top of a blank plastic block, cut on an ultramicrotome, and collected on grids for viewing with the electron microscope.
The advantages of this method are quick identification of the precise location of the area of interest and permanent slides for further studies. This procedure will enable the researcher to perform a maximum number tests with a minimum of tissue.