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Abstract
A rapid silver method to demonstrate glomerular basement membranes is described. The method uses the dilute ammoniacal silver solution as described by Churukian et al. for the demonstration of fungus and Pneumocystis carinii. The ammoniacal silver solution replaces the silver methenamine solution in the modified Jones' method for basement membranes. The heat needed to impregnate the basement membranes with silver is provided by the microwave oven, thus decreasing the total staining time. The staining results are consistent, and each glomerulus throughout the biopsy is evenly stained with no background silver debris. Renal biopsies fixed in both Helly's and 10% buffered formalin are stained equally well. (The J Histotechnol 12:105, 1989.)