Stable Trichrome for the Demonstration of the Intermyofibrillary Network in Muscle Biopsies

Abstract

The modified Gomori trichrome, pH 3.4, has been useful in the visualization of the intermyofibrillary network, which is not obvious by connective tissue techniques of the Masson type. However, it is often capricious, requiring fresh preparation before use. The method presented, which uses three stock buffered stain solutions of the required pH, clearly demonstrates both the tissue and cell structure and has been reliably used in the diagnoses of over 600 human muscle biopsies. The solution is easily prepared and remains stable for several months. (The J Histotechnol 17:59, 1990)

Keywords:

• frozen muscle biopsy
• intermyofibrillary network
• muscle
• trichrome