Tissue Transfer Technique for Transferring Animal Tissues onto Membrane Substrates for Rapid Histological Evaluation

Abstract

A method is described for transferring and immobilizing an intact tissue layer from animal tissues to the surface of membrane substrates for the purpose of histological evaluation. Using rabbit kidney as a prototype, tissue transfers were examined either by light microscopy (after staining with hematoxylin and eosin and mounting on glass slides) or by transmission electron microscopy (after embedding and sectioning). Both types of preparations demonstrated architectural preservation at the organ, tissue, and cellular level. The characteristics of tissue transfers suggests that they are a useful adjunct to routine histological techniques particularly in circumstances where speed of preparation, cost, large sample size, and exploration of cell surfaces is advantageous (The J Histotechnol 18:307, 1995).

Keywords:

• animal tissue
• membrane structures
• tissue transfer