Fluorochrome Labeled Bone (Part II): Results From Various Slide Preparation Techniques and Microscopic Filters

Abstract

Laboratory technicians have been preparing bone samples for fluorochrome viewing since the late 1950s. Improvements in technique and advancements in materials have enabled specimen processing and preparation to elevate to a higher standard of quality. However, there is no set of standards with which to guide the novice in preparing slides for subsequent microscopic observation. This study reports results from 15 slide comparison methods. While many of the slide preparation techniques yielded similar results to the control slide, several methods including three staining procedures, were substandard for optimal fluorochrome viewing. (The J Histotechnol 25:103, 2002)

Submitted March 15, 2002; accepted April 5, 2002

Keywords:

• cyanoacrylate
• fluorochrome
• Technovits®