New Staphylococcus aureus Incompatibility Group 1 Plasmids Encoding Penicillinase roduction and Resistance to Different Antibacterial Agents

Abstract

Eleven Staphylococcus aureus plasmids encoding penicillinase production and resistance to different antibacterial agents were transferred to laboratory recipient strains in mixed-culture transfer and transduction experiments and characterized by restriction endonuclease analysis and incompatibility. The plasmids were differentiated into four types (types A-D) on the basis of their resistance phenotypes and restriction endonuclease patterns. One type encoded resistance to cadmium and arsenate. The second type encoded resistance to cadmium, mercuric compounds and nucleic acid-binding compounds. The third type encoded resistance to cadmium, kanamycin, neomycin and streptomycin while the fourth type encoded resistance to kanamycin, neomycin and ethidium bromide. Plasmids within the same class were structurally related or similar and were different from those in the other classes. Three plasmids, pWBG626, pWBG628, and pWBG663, representing three of the four plasmid types, belonged to incompatibility group 1. These new plasmids add to the number of known incompatibility group 1 plasmids and have resistance phenotypes which should be useful for studying incompatibility of new S. aureus plasmids.