Intravenous 2-Chloroadenosine Protects BALB/C Mice from Klebsiella pneumoniae B5055-Induced Sepsis by Modulating the Pro-Inflammatory Immune Response

Abstract

Sepsis is a severe inflammatory immune response of the host against an infectious agent or its product i.e. lipopolysaccharide (LPS). Therefore, targeting the immune system during sepsis may lower the morbidity and mortality associated with sepsis. The present study shows the protective immunomodulatory action of 2-chloroadenosine (2-CADO) in K. pneumoniae B 5055 induced sepsis in male BALB/c mice. Sepsis was induced by implanting the fibrin-thrombin clot containing known amount (10²cfu) of Klebsiella pneumoniae B5055 into the peritoneal cavity of mice. 100% mortality with in the 5 post infection days (PIDs) was observed in control group animals. Intravenous 2-CADO (10 μg/kg/day) treatment increased in survival of animals by 70% without significantly (p>0.05) decreasing the blood bacterial load. But a significant (p<0.05) decrease in the level of inflammatory markers (TNF-α, IL-1α, MDA, NO) responsible for sepsis was observed. However, serum IL-10 levels were found to be significantly (p<0.05) increased with 2-CADO treatment.

Keywords:

• 2-chloroadenosine (2-CADO)
• sepsis
• TNF-α
• IL-10
• IL-1α
• MDA
• NO
• Klebsiella pneumoniae B5055