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Original Articles

Development of a Gene Transfer System for the Mycelia of Flammulina velutipes Fv-1 Strain

, , , , , & show all
Pages 1126-1128 | Received 12 Jan 2010, Accepted 11 Feb 2010, Published online: 22 May 2014
 

Abstract

To develop a gene transformation method for Flammulina velutipes, we constructed a vector with hph gene under control of the trp1 gene promoter. The vector was integrated into protoplast derived from mycelia by the calcium-polyethylene glycol method, as it has not been reported for F. velutipes. Transformation efficiency was much improved when transformation was performed by the restriction enzyme mediated integration method.

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