Effects of Modification of Amino Groups with Poly(Ethylene Glycol) on a Recombinant Uricase from Bacillusfastidiosus

Chun ZHANGUnit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University

Xiaolan YANGUnit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University

Juan FENGUnit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University

Yonghua YUANUnit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University

Xiang LIUnit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University

Youquan BUUnit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University

Yanling XIEUnit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University

Huidong YUANUnit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University

Fei LIAOUnit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University

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Abstract

After modification with monomethoxyl-poly(ethylene glycol)-5000, a recombinant intracellular uricase from Bacillus fastidiosus ATCC 29604 showed residual activity of about 65%, a thermo-inactivation half-life >85 h, a circulating half-life about 20 h in rats in vivo, consistent effects of common cations, and consistent optima for reaction temperature and pH. Thus, this uricase can be formulated via modification with monomethoxyl-poly(ethylene glycol).

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Effects of Modification of Amino Groups with Poly(Ethylene Glycol) on a Recombinant Uricase from Bacillusfastidiosus

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Effects of Modification of Amino Groups with Poly(Ethylene Glycol) on a Recombinant Uricase from Bacillusfastidiosus

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