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Bioscience, Biotechnology, and Biochemistry Volume 74, 2010 - Issue 6
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Original Articles

Gateway Binary Vectors with the Bialaphos Resistance Gene, bar, as a Selection Marker for Plant Transformation

Shinya NAKAMURADepartment of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
,
Shoji MANODepartment of Cell Biology, National Institute for Basic Biology;Department of Basic Biology, School of Life Science, The Graduate University for Advanced Studies
,
Yuji TANAKADepartment of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
,
Masato OHNISHIDepartment of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
,
Chihiro NAKAMORIDepartment of Cell Biology, National Institute for Basic Biology
,
Masami ARAKIDepartment of Cell Biology, National Institute for Basic Biology
,
Tomoko NIWADepartment of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
,
Mikio NISHIMURADepartment of Cell Biology, National Institute for Basic Biology;Department of Basic Biology, School of Life Science, The Graduate University for Advanced Studies
,
Hironori KAMINAKADepartment of Bioresource Sciences, Faculty of Agriculture, Tottori University
,
Tsuyoshi NAKAGAWADepartment of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
,
Yutaka SATODepartment of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University;PRESTO, Japan Science and Technology Agency
&
Sumie ISHIGURODepartment of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
 show all
Pages 1315-1319 | Received 15 Mar 2010, Accepted 07 May 2010, Published online: 22 May 2014
 
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Abstract

We constructed two series of Gateway binary vectors, pGWBs and R4pGWBs, possessing the bialaphos resistance gene (bar) as a selection marker for plant transformation. The reporters and tags employed in this system are sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, TagRFP, 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7 and TAP. Selection of Arabidopsis transformants with BASTA® was successfully carried out using both plate-grown and soil-grown seedlings. Transformed rice calli and suspension-cultured tobacco cells were selected on plates containing BASTA® or glufosinate-ammonium. These vectors are compatible with existing pGWB and R4pGWB vectors carrying kanamycin and hygromycin B resistance.

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