A Yeast Bioassay for Androgenic and Anti-Androgenic Compounds Based on the NH₂- and COOH-Terminal Interaction of Androgen Receptor

Abstract

Androgenic compounds induce an interaction between the NH₂- and COOH-terminal regions (N–C interaction) of androgen receptor (AR). We describe a rapid yeast bioassay for androgenic and anti-androgenic compounds based on androgen-dependent β-catenin-enhanced N–C interaction. The bioassay was also effective at detecting compounds that inhibit the N–C interaction in ways that do not involve binding to the ligand-binding domain.

• androgen receptor (AR)
• bioassay
• N–C interaction
• β-catenin
• yeast two-hybrid