Characterization of Paenibacillus curdlanolyticus Intracellular Xylanase Xyn10B Encoded by the xyn10B Gene

Maya SUDOApplied Microbiology Laboratory, Graduate School of Bioresources, Mie University

Makiko SAKKAApplied Microbiology Laboratory, Graduate School of Bioresources, Mie University

Tetsuya KIMURAApplied Microbiology Laboratory, Graduate School of Bioresources, Mie University

Khanok RATANAKHANOKCHAISchool of Bioresources and Technology, King Mongkut’s University of Technology Thonburi

Kazuo SAKKAApplied Microbiology Laboratory, Graduate School of Bioresources, Mie University

Abstract

The Paenibacillus curdlanolyticus xyn10B gene encoding a family-10 xylanase was cloned and expressed in Escherichia coli, and the recombinant enzyme rXyn10B was characterized. Immunological analysis suggested that Xyn10B is an intracellular enzyme. rXyn10B hydrolyzed birch-wood xylan and xylooligosaccharides to produce mainly xylobiose, suggesting that it is an endoxylanase. Its properties were significantly different from those of some homologous enzymes.

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Characterization of Paenibacillus curdlanolyticus Intracellular Xylanase Xyn10B Encoded by the xyn10B Gene

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Characterization of Paenibacillus curdlanolyticus Intracellular Xylanase Xyn10B Encoded by the xyn10B Gene

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