Characterization of an Aspergillus oryzae Cysteinyl Dipeptidase Expressed in Escherichia coli

Ryota HATTORINational Food Research Institute, National Agriculture and Food Research Organization (NARO)

Mayumi MATSUSHITA-MORITANational Food Research Institute, National Agriculture and Food Research Organization (NARO)

Junichiro MARUINational Food Research Institute, National Agriculture and Food Research Organization (NARO)

Sawaki TADANational Food Research Institute, National Agriculture and Food Research Organization (NARO)

Satoshi SUZUKINational Food Research Institute, National Agriculture and Food Research Organization (NARO)

Ikuyo FURUKAWANational Food Research Institute, National Agriculture and Food Research Organization (NARO)

Youhei YAMAGATADepartment of Agriscience and Bioscience, Tokyo University of Agriculture and Technology

Hitoshi AMANOGifu R&D Center, Amano Enzyme Inc.

Hiroki ISHIDAResearch Institute, Gekkeikan Sake Company Ltd.

Michio TAKEUCHIDepartment of Agriscience and Bioscience, Tokyo University of Agriculture and Technology

Ken-Ichi KUSUMOTONational Food Research Institute, National Agriculture and Food Research Organization (NARO)

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Abstract

Cysteinyl dipeptidase from Aspergillus oryzae (CdpA) was produced in Escherichia coli and purified. The enzyme showed activity specific toward cysteine-containing dipeptides, but its substrate specificity was distinct from those of other cysteinyl dipeptidases of the M20 family. It was optimally active at pH 7–8 and stable at pH 6–9 and at up to 40 °C.

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Characterization of an Aspergillus oryzae Cysteinyl Dipeptidase Expressed in Escherichia coli

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Characterization of an Aspergillus oryzae Cysteinyl Dipeptidase Expressed in Escherichia coli

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