Heterologous Expression of Aspergillus oryzae Xylose Reductase and Xylitol Dehydrogenase Genes Facilitated Xylose Utilization in the Yeast Saccharomyces cerevisiae

Jitsuro KANEDADepartment of Bioindustrial Informatics and Genomics, Graduate School of Agricultural Science, Tohoku University

Kenji SASAKIDepartment of Bioindustrial Informatics and Genomics, Graduate School of Agricultural Science, Tohoku University

Katsuya GOMIDepartment of Bioindustrial Informatics and Genomics, Graduate School of Agricultural Science, Tohoku University

Takahiro SHINTANIDepartment of Bioindustrial Informatics and Genomics, Graduate School of Agricultural Science, Tohoku University

Abstract

The cDNA encoding a putative xylose reductase (xyrA) from Aspergillus oryzae was cloned and coexpressed in the yeast Saccharomyces cerevisiae with A. oryzae xylitol dehydrogenase cDNA (xdhA). XyrA exhibited NADPH-dependent xylose reductase activity. The S. cerevisiae strain, overexpressing the xyrA, xdhA, endogenous XKS1, and TAL1 genes, grew on xylose as sole carbon source, and produced ethanol.

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Heterologous Expression of Aspergillus oryzae Xylose Reductase and Xylitol Dehydrogenase Genes Facilitated Xylose Utilization in the Yeast Saccharomyces cerevisiae

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Heterologous Expression of Aspergillus oryzae Xylose Reductase and Xylitol Dehydrogenase Genes Facilitated Xylose Utilization in the Yeast Saccharomyces cerevisiae

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