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Original Articles

Characterization of Recombinant β-Amylases from Oryza sativa

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Pages 793-796 | Received 07 Dec 2010, Accepted 17 Jan 2011, Published online: 22 May 2014
 

Abstract

Four putative β-amylase genes found in the Oryza sativa cDNA sequence database (KOME) were expressed in Escherichia coli. Recombinant proteins from two of these genes showed β-amylase activity. Similarly to β-amylases from other plants, the optimum pH of the recombinant rice β-amylases was about 5.5–6.0, but they exhibited inferior heat stability to soybean β-amylase.

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