Polyhydroxyalkanoate (PHA) Synthesis by Class IV PHA Synthases Employing Ralstonia eutropha PHB⁻4 as Host Strain

Manami HYAKUTAKEDepartment of Innovative and Engineered Materials, Tokyo Institute of Technology

Yuta SAITODepartment of Innovative and Engineered Materials, Tokyo Institute of Technology

Satoshi TOMIZAWADepartment of Innovative and Engineered Materials, Tokyo Institute of Technology

Kouhei MIZUNODivision of Biochemical Engineering, Department of Materials Science and Chemical Engineering, Kitakyushu National College of Technology

Takeharu TSUGEDepartment of Innovative and Engineered Materials, Tokyo Institute of Technology

Abstract

Class IV polyhydroxyalkanoate (PHA) synthase from Bacillus cereus YB-4 (PhaRC_YB4) or B. megaterium NBRC15308^T (PhaRC_Bm) was expressed in Ralstonia eutropha PHB⁻4 to compare the ability to produce PHA and the substrate specificity of PhaRCs. PhaRC_YB4 produced significant amounts of PHA and had broader substrate specificity than PhaRC_Bm.

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Polyhydroxyalkanoate (PHA) Synthesis by Class IV PHA Synthases Employing Ralstonia eutropha PHB⁻4 as Host Strain

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Polyhydroxyalkanoate (PHA) Synthesis by Class IV PHA Synthases Employing Ralstonia eutropha PHB−4 as Host Strain

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Polyhydroxyalkanoate (PHA) Synthesis by Class IV PHA Synthases Employing Ralstonia eutropha PHB⁻4 as Host Strain