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Original Articles

TPS1 Terminator Increases mRNA and Protein Yield in a Saccharomyces cerevisiae Expression System

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Pages 2234-2236 | Received 29 Mar 2011, Accepted 29 Jul 2011, Published online: 22 May 2014
 

Abstract

Both terminators and promoters regulate gene expression. In Saccharomyces cerevisiae, the TPS1 terminator (TPS1t), coupled to a gene encoding a fluorescent protein, produced more transgenic mRNA and protein than did similar constructs containing other terminators, such as CYC1t, TDH3t, and PGK1t. This suggests that TPS1t can be used as a general terminator in the development of metabolically engineered yeast in high-yield systems.

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