Overexpression of the (R)-Specific Enoyl-CoA Hydratase Gene from Pseudomonas chlororaphis HS21 in Pseudomonas Strains for the Biosynthesis of Polyhydroxyalkanoates of Altered Monomer Composition

Abstract

The (R)-specific enoyl-CoA hydratase gene (phaJ _HS21) from Pseudomonas chlororaphis HS21 was overexpressed in various Pseudomonas strains, alone and in combination with the polyhydroxyalkanoate synthase gene (phaC _HS21), for the biosynthesis of polyhydroxyalkanoates (PHAs) of altered monomer composition. Recombinant Pseudomonas strains harboring phaC _HS21 and phaJ _HS21 generated saturated and unsaturated monomers of C12–C14 in their PHAs. In particular, the level of the 3-hydroxytetradecenoate monomer in recombinant P. chlororaphis HS21 increased by approximately 260%. PhaJ_HS21 is expected to be useful in the biosynthesis of PHAs consisting of unusual monomer units.

• monomer composition
• PHA synthase
• polyhydroxyalkanoates
• Pseudomonas chlororaphis HS21
• (R)-specific enoyl-CoA hydratase