The carbohydrate-binding activity of lectins was examined using polyamidoamine dendrimer conjugated with carbohydrates (sugar-PD). When a C-type lectin, CEL-IV, was mixed with melibiose-PD, large complexes with a diameter of about 1 μm were formed. Changes in the amount of CEL-IV/melibiose-PD complex as an indication of lectin activity were measured sensitively by Rayleigh scattering. The carbohydrate specificity of the lectin was determined on the basis of inhibition of complex-formation by individual carbohydrates. It is suggested that various lectins can also be measured using sugar-PDs to which different carbohydrates are attached.
Full access
An Assay for Carbohydrate-Binding Activity of Lectins Using Polyamidoamine Dendrimer Conjugated with Carbohydrates
Reprints and Corporate Permissions
Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?
To request a reprint or corporate permissions for this article, please click on the relevant link below:
Academic Permissions
Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?
Obtain permissions instantly via Rightslink by clicking on the button below:
If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.
Related research
People also read lists articles that other readers of this article have read.
Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.
Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.