A Rapid and Economical Method for Low Molecular Weight RNA Isolation from a Wide Variety of Plant Species

Zewei ANCollege of Agriculture, Hainan University;State Center for Rubber Breeding and Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences

Yachao LICollege of Agriculture, Hainan University

Lili XIECollege of Agriculture, Hainan University

Qilin ZHAICollege of Agriculture, Hainan University

Huasun HUANGState Center for Rubber Breeding and Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences

Abstract

We have developed a time- and cost-effective method for isolating low molecular weight (LMW) RNA from plants. In our protocol, the isolation procedure can be completed within 3 h. Polyethylene glycol (PEG) and absolute ethanol are used to isolate LMW RNA, and the LMW RNA yields were >80 µg/g of fresh-weight tissues for several of the plant species tested.

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A Rapid and Economical Method for Low Molecular Weight RNA Isolation from a Wide Variety of Plant Species

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A Rapid and Economical Method for Low Molecular Weight RNA Isolation from a Wide Variety of Plant Species

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