Abstract
The fruit body formation of Schizophyllum commune was greatly stimulated by the addition of papulacandin B, aculeacin A, gramicidin S, luteinizing hormone-releasing hormone (LH-RH), or digitonin to the culture media. Papulacandin B and aculeacin A inhibit glucan synthase, which was demonstrated with S. commune in vitro in this study. The in vitro S. commune glucan synthase was activated by phospholipids that counteracted the stimulation of fruit body formation by papulacandin B. LH-RH, however, did not inhibit the glucan synthase. Digitonin was known to inhibit chitin synthase, but polyoxin B, a substrate analogue inhibitor of chitin synthase, did not stimulate the fruit body formation. The characteristic common to most of these stimulators of fruit body formation is possible induction of structural changes of plasma membranes to which the substances can attach with their biphasic nature. These stimulators of fruit body formation were mostly accompanied by an evident suppression of hyphal growth, independent of phenoloxidase activity, and not observed in continuous dark or with monokaryotic strains.