Enzymatic Assay for Adenosylcobalamin-dependent Methylmalonyl Coenzyme A Mutase

Fumio WatanabeLaboratory of Nutrition and Food Science, Hagoromo-gakuen College, 1–89–1 Hamadera-minamimachi, Sakai, Osaka592, Japan

Yoshiyuki TamuraLaboratory of Nutrition and Food Science, Hagoromo-gakuen College, 1–89–1 Hamadera-minamimachi, Sakai, Osaka592, Japan

Hisako SaidoLaboratory of Nutrition and Food Science, Hagoromo-gakuen College, 1–89–1 Hamadera-minamimachi, Sakai, Osaka592, Japan;Department of Agricultural Chemistry, University of Osaka Prefecture, Sakai, Osaka593, Japan

Yoshihisa NakanoLaboratory of Nutrition and Food Science, Hagoromo-gakuen College, 1–89–1 Hamadera-minamimachi, Sakai, Osaka592, Japan;Department of Agricultural Chemistry, University of Osaka Prefecture, Sakai, Osaka593, Japan

Abstract

An enzymatic method for assaying methylmalonyl-CoA mutase was devised. The method employs a succinyl-CoA transferase and β-hydroxyacyl-CoA dehydrogenase-coupled reaction to make the formation of NAD⁺ stoichiometric with the amount of the succinyl-CoA formed by the mutase. This method is one of the most convenient and useful assays for the activity of methylmalonyl-CoA mutase in biological samples.

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Enzymatic Assay for Adenosylcobalamin-dependent Methylmalonyl Coenzyme A Mutase

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Enzymatic Assay for Adenosylcobalamin-dependent Methylmalonyl Coenzyme A Mutase

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