Purification and Characterization of Cytochrome P450 from an Isobutene-forming Microorganism, Rhodotorula minuta

Hideo FukudaDepartment of Applied Microbial Technology, The Kumamoto Institute of Technology, Ikeda 4–22–1, Kumamoto860, Japan

Takao FujiiDepartment of Applied Microbial Technology, The Kumamoto Institute of Technology, Ikeda 4–22–1, Kumamoto860, Japan

Hironori DaimonDepartment of Applied Microbial Technology, The Kumamoto Institute of Technology, Ikeda 4–22–1, Kumamoto860, Japan

Makoto IwataDepartment of Applied Microbial Technology, The Kumamoto Institute of Technology, Ikeda 4–22–1, Kumamoto860, Japan

Takahira OgawaDepartment of Applied Microbial Technology, The Kumamoto Institute of Technology, Ikeda 4–22–1, Kumamoto860, Japan

Sumio TanaseDepartment of Applied Microbial Technology, The Kumamoto Institute of Technology, Ikeda 4–22–1, Kumamoto860, Japan;Department of Biochemistry, Kumamoto University School of Medicine, Honjo 2–2–1, Kumamoto860, Japan

Yoshimasa MorinoDepartment of Applied Microbial Technology, The Kumamoto Institute of Technology, Ikeda 4–22–1, Kumamoto860, Japan;Department of Biochemistry, Kumamoto University School of Medicine, Honjo 2–2–1, Kumamoto860, Japan

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Abstract

A cytochrome P450 was purified from microsomes of Rhodotorula minuta. The optical spectrum of the purified cytochrome was characteristic of a low-spin ferric heme protein. Isovalerate caused a type I spectral change in it. The amino-terminal sequence of the cytochrome was different from those of other known microsomal cytochrome P450s. These results indicate that the cytochrome, which is tentatively named P450rm, is a novel species of cytochrome P450.

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Purification and Characterization of Cytochrome P450 from an Isobutene-forming Microorganism, Rhodotorula minuta

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Purification and Characterization of Cytochrome P450 from an Isobutene-forming Microorganism, Rhodotorula minuta

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