Improved Method for Purification of Leukocidin and Gamma-Hemolysin Components from Staphylococcus aureus

Hirofumi NariyaDepartment of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Amamiya-machi, Aoba-ku, Sendai981, Japan

Ikuno AsamiDepartment of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Amamiya-machi, Aoba-ku, Sendai981, Japan

Toshiko OzawaDepartment of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Amamiya-machi, Aoba-ku, Sendai981, Japan

Yoshinori BeppuDepartment of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Amamiya-machi, Aoba-ku, Sendai981, Japan

Kazuo IzakiDepartment of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Amamiya-machi, Aoba-ku, Sendai981, Japan

Yoshiyuki KamioDepartment of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Amamiya-machi, Aoba-ku, Sendai981, Japan

Abstract

For the purification of the F and S components of leukocidin, and the HγII component of γ-hemolysin from methicillin resistant taphylococcus aureus RIMD 310925, an easy two-step method was developed. This technique yielded a recovery of 3.6, 1.6, and 6.4mg of purified F, S, and HγII, respectively, per liter of the culture supernatant with a recovery of more than 90% each from the culture supernatant.

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Improved Method for Purification of Leukocidin and Gamma-Hemolysin Components from Staphylococcus aureus

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Improved Method for Purification of Leukocidin and Gamma-Hemolysin Components from Staphylococcus aureus

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