Isolation of a Chitin Synthase Gene (chsC) of Aspergillus nidulans

Takayuki MotoyamaDepartment of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo113, Japan

Nobuko KojimaDepartment of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo113, Japan

Hiroyuki HoriuchiDepartment of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo113, Japan

Akinori OhtaDepartment of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo113, Japan

Masamichi TakagiDepartment of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo113, Japan

Abstract

We isolated a class I chitin synthase gene (chsC) from Aspergillus nidulans. Expression of this gene was confirmed by Northern analysis and by sequencing of the PCR-amplified DNA fragments from cDNA. chsC disruptants showed no difference of morphology in the asexual cycle and no difference of growth rate compared to a wild-type strain.

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Isolation of a Chitin Synthase Gene (chsC) of Aspergillus nidulans

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Isolation of a Chitin Synthase Gene (chsC) of Aspergillus nidulans

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