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Bioscience, Biotechnology, and Biochemistry Volume 70, 2006 - Issue 9
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Original Articles

Posttranscriptional Regulation by the Upstream Open Reading Frame of the Phosphoethanolamine N-Methyltransferase Gene

Tomoki TABUCHILaboratory of Tropical Plant Science, Faculty of Agriculture, Kobe University
,
Tomoyuki OKADALaboratory of Tropical Plant Science, Faculty of Agriculture, Kobe University
,
Tetsushi AZUMAGraduate School of Science and Technology, Kobe University
,
Takashi NANMORIGraduate School of Science and Technology, Kobe University
&
Takeshi YASUDALaboratory of Tropical Plant Science, Faculty of Agriculture, Kobe University;Graduate School of Science and Technology, Kobe University
Pages 2330-2334 | Received 06 Jun 2006, Accepted 20 Jul 2006, Published online: 22 May 2014
 
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Abstract

Phosphoethanolamine N-methyltransferase (PEAMT) is involved in choline biosynthesis in plants. The 5′ untranslated region (UTR) of several PEAMT genes was found to contain an upstream open reading frame (uORF). We generated transgenic Arabidopsis calli that expressed a chimeric gene constructed by fusing the 5′ UTR of the Arabidopsis PEAMT gene (AtNMT1) upstream of the β-glucuronidase gene. The AtNMT1 uORF was found to be involved in declining levels of the chimeric gene mRNA and repression of downstream β-glucuronidase gene translation in the calli when the cells were treated with choline. Further, we discuss the role of the uORF.

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Related Research Data

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