Isolation and Characterization of Chitinase from a Flake-chitin Degrading Marine Bacterium, Aeromonas hydrophila H-2330

Kazumi HiragaDepartment of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology, Sakyo-ku, Kyoto606, Japan

Lee ShouDepartment of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology, Sakyo-ku, Kyoto606, Japan

Mitsunori KitazawaDepartment of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology, Sakyo-ku, Kyoto606, Japan

Saori TakahashiDepartment of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology, Sakyo-ku, Kyoto606, Japan

Masahiko ShimadaCentral Research Institute, Maruha, Tsukuba300-42, Japan;Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology, Sakyo-ku, Kyoto606, Japan

Ryoichi SatoCentral Research Institute, Maruha, Tsukuba300-42, Japan;Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology, Sakyo-ku, Kyoto606, Japan

Kohei OdaDepartment of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology, Sakyo-ku, Kyoto606, Japan

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Abstract

A flake-chitin degrading marine bacterium was isolated and identified as Aeromonas hydrophila. This strain secreted five chitinases and an β-N-acetylglucosaminidase. The main chitinase (Chi-A) was purified and characterized. The optimum pH of Chi-A was 5–8, and the activity was inhibited by Hg²⁺ and Fe³⁺. Chi-A was different from chitinases of other Aeromonas species with respect to molecular weight (62, 000) and insensitivity to monoiodoacetate. The amino-terminal amino acid sequence showed extensive similarity with chitinases from Gram-negative bacteria.

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Isolation and Characterization of Chitinase from a Flake-chitin Degrading Marine Bacterium, Aeromonas hydrophila H-2330

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Isolation and Characterization of Chitinase from a Flake-chitin Degrading Marine Bacterium, Aeromonas hydrophila H-2330

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