Micro-assay Method for Evaluating the Allergenicity of the Major Soybean Allergen, Gly m Bd 30K, with Mouse Antiserum and RBL-2H3 Cells

Rintaro YamanishiDepartment of Nutrition, School of Medicine, The University of Tokushima, 3–18–15 Kuramoto-cho, Tokushima 770, Japan

Hideaki TsujiDepartment of Nutrition, School of Medicine, The University of Tokushima, 3–18–15 Kuramoto-cho, Tokushima 770, Japan

Noriko BandoDepartment of Nutrition, School of Medicine, The University of Tokushima, 3–18–15 Kuramoto-cho, Tokushima 770, Japan

Izumi YoshimotoDepartment of Nutrition, School of Medicine, The University of Tokushima, 3–18–15 Kuramoto-cho, Tokushima 770, Japan

Tadashi OgawaDepartment of Nutrition, School of Medicine, The University of Tokushima, 3–18–15 Kuramoto-cho, Tokushima 770, Japan

Abstract

A micro-assay method for evaluating the allergenicity of soybean allergen was developed by using the mouse antiserum against Gly m Bd 30K, a major soybean allergen, and RBL-2H3, a rat mucosal mast cell line. The antiserum against Gly m Bd 30K was prepared by subcutaneously immunizing BALB/c mice with the allergen. The behavior by affinity-chromatography and the properties against heat treatment show that the reaginic activity of the antiserum resided in the IgE antibody specific for Gly m Bd 30K. The developed assay method is shown to be useful for simulating IgE mediated type-I allergy and to be highly sensitive for detecting the allergen.

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Micro-assay Method for Evaluating the Allergenicity of the Major Soybean Allergen, Gly m Bd 30K, with Mouse Antiserum and RBL-2H3 Cells

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Micro-assay Method for Evaluating the Allergenicity of the Major Soybean Allergen, Gly m Bd 30K, with Mouse Antiserum and RBL-2H3 Cells

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