Coulometric Electrochemical Detection of Phospholipid Hydroperoxides by High-performance Liquid Chromatography

Hirofumi AraiDepartment of Marine Bioresources Chemistry, Faculty of Fisheries, Hokkaido University, Hakodate, Hokkaido041, Japan

Satoshi MohriDepartment of Marine Bioresources Chemistry, Faculty of Fisheries, Hokkaido University, Hakodate, Hokkaido041, Japan;Miyagi Prefectural Institute of Technology, Sendai, Miyagi982, Japan

Tetsuya SuzukiDepartment of Marine Bioresources Chemistry, Faculty of Fisheries, Hokkaido University, Hakodate, Hokkaido041, Japan

Kozo TakamaDepartment of Marine Bioresources Chemistry, Faculty of Fisheries, Hokkaido University, Hakodate, Hokkaido041, Japan

Junji TeraoDepartment of Marine Bioresources Chemistry, Faculty of Fisheries, Hokkaido University, Hakodate, Hokkaido041, Japan;National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, Tsukuba, Ibaraki305, JapanCorrespondence[email protected]

Abstract

A technique for the sensitive and convenient determination of phosphatidylcholine hydroperoxides was developed that uses coulometric electrochemical detection in a high-performance liquid chromatographic analysis. The detection limit for phosphatidylcholine hydroperoxides by this method was 20 pmol at a signal-to-noise ratio of 3. The glutathione-dependent phospholipid hydroperoxide-reducing activity of fresh human plasma was ascertained by this method.

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Coulometric Electrochemical Detection of Phospholipid Hydroperoxides by High-performance Liquid Chromatography

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Coulometric Electrochemical Detection of Phospholipid Hydroperoxides by High-performance Liquid Chromatography

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