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Abstract
A microorganism able to grow in mineral salt medium with styrene (0.1–10%) as a sole carbon source was isolated. The bacterium belongs to the genus Pseudomonas. Pseudomonas LF-3 used not only styrene but also toluene as a carbon source. Cell viability, total cell count, and turbidity with 0.1% showed good results. Cell viability reached 7 × 108 cells/ml with 1.0% styrene and remained near this after reaching its maximum. Maximum cell viability was 1.4 × 106 cells/ml with 1.0% styrene and 1.1 × 106 cells/ml with 10% styrene, respectively. Pseudomonas LF-3 grew on 1-phenylethanol, 2-phenylethanol, benzylalcohol, acetophenone, phenyl-1,2-ethanediol, benzoic acid, or protocatechuic acid. The degradation product from styrene was separated and identified as 3-vinylcatechol. The yellow color observed in the medium suggests a meta ring cleavage pathway.