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Abstract
The effects of cholestane 3β,5α,6β-triol on intestinal epithelial crypt cells were investigated using the IEC-6 cell line. Cholestane 3β,5α,6β-triol decreased SH groups (glutathione and protein SH) in the cell, and showed cytotoxicity in a time-dependent manner. Although the concentration of cholestane 3β,5α,6β-triol used in this study (100μm) was very high compared with that in plasma of experimental animals, cholestane 3β,5α,6β-triol did not show any cytotoxicity on IEC-6 cells without fetal calf serum (FCS). The level of cytotoxicity was dependent upon the concentration of FCS in the culture medium. Unknown components in FSC (not VLDL or LDL) were suggested to be associated with the cytotoxicity of cholestane 3β,5α,6β-triol. Moreover, the fact that even heat-treated FCS (100°C for 30 min) still mediated the cytotoxicity suggested the participation of non-protein components.