Identification of a Structural Gene Encoding a Metallothionein-like Domain that Includes a Putative Regulator Protein for Streptomyces Protease Gene Expression

Abstract

An open reading frame (termed ORF-PR) encoding a metallothionein-like domain-including protein was found upstream of a previously identified Streptomyces chymotrypsin-type protease gene (sam-P20). Promoter and terminator activities of ORF-PR were detected using the promoterless Streptomyces tyrosinase gene as a reporter gene and expression of ORF-PR was supposed to occur before that of sam-P20 gene. Frameshift mutation anaysis showed that the ORF-PR product might act as a repressive regulator of the sam-P20 gene.

• Streptomyces
• metallothionein-like domain
• sequence comparison
• DNA-binding motif
• protease gene