A Non-radioassay of Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase by Anion-Exchange Chromatography

Koichi UEMURAPlant Molecular Physiology Laboratory, Research Institute of Innovative Technology for the Earth (RITE)

Ayako SHIMOIDEPlant Molecular Physiology Laboratory, Research Institute of Innovative Technology for the Earth (RITE)

Akiho YOKOTAPlant Molecular Physiology Laboratory, Research Institute of Innovative Technology for the Earth (RITE);Graduate School of Biological Sciences, Nara Institute of Science and Technology (NAIST)

Abstract

A non-radioisotopic assay method for ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is described. 3-Phosphoglycerate produced by the RuBisCO reaction was measured using an anion-exchange chromatography. The reaction course of spinach RuBisCO measured by this method was completely the same as that measured with ¹⁴CO₂.

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A Non-radioassay of Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase by Anion-Exchange Chromatography

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A Non-radioassay of Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase by Anion-Exchange Chromatography

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