Abstract
Anthocyanin-producing cells in the tepals of flower buds of Hyacinthus orientalis were investigated. In field-grown flowers, anthocyanins were only produced in L2 cells (cells in the sub-epidermis) of tepals. However, when early-stage flower buds were cultured on medium containing GA3, anthocyanins were produced not only in L2 cells but also in L1 cells (cells in the epidermal layer) of tepals. The same anthocyanins were produced in tepals of both field-grown flowers and flower buds cultured in vitro.