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Bioscience, Biotechnology, and Biochemistry Volume 64, 2000 - Issue 6
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Original Articles

New Insecticidal Bufadienolide, Bryophyllin C, from Kalanchoe pinnata

Unang SUPRATMANDepartment of Applied Biological Chemistry, College of Agriculture, Osaka Prefecture University
,
Tomoyuki FUJITADepartment of Applied Biological Chemistry, College of Agriculture, Osaka Prefecture University
,
Kohki AKIYAMADepartment of Applied Biological Chemistry, College of Agriculture, Osaka Prefecture University
&
Hideo HAYASHIDepartment of Applied Biological Chemistry, College of Agriculture, Osaka Prefecture University
Pages 1310-1312 | Received 06 Dec 1999, Accepted 31 Jan 2000, Published online: 22 May 2014
 
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Abstract

Two insecticidal bufadienolides (1 and 2) were isolated from a methanol extract of the leaves of Kalanchoe pinnata by bioassay-guided fractionation. Compound 1 was identified as known bryophyllin A (bryotoxin C). The structure of new bufadienolide 2, named bryophyllin C, was determined by spectroscopic methods and the chemical transformation of 1. Compounds 1 and 2 showed strong insecticidal activity against third instar larvae of the silkworm (Bombyx mori), their LD50 values being evaluated as 3 and 5 μg/g of diet, respectively.

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