The aman6 Gene Encoding a Yeast Mannan Backbone Degrading 1,6-α-D-Mannanase in Bacillus circulans: Cloning, Sequence Analysis, and Expression

Abstract

A gene (aman6) encoding endo-1,6-α-D-mannanase, a yeast mannan backbone degrading enzyme from Bacillus circulans was cloned. The putative aman6 was 1767 base pairs long and encoded a mature 1,6-α-D-mannanase protein of 589 amino acids and a signal peptide of 36 amino acids. The purified mature 1,6-α-D-mannanase from the Escherichia coli transformant showed 61-kDa protein, and N-terminal amino acid sequence and other general properties of the recombinant enzyme were identical to those of 1,6-α-D-mannanase from Bacillus circulans TN-31.

• yeast mannoprotein
• 1,6-α-D-mannanase
• recombinant enzyme
• aman6 gene
• Bacillus circulans