Detection of Biomarkers for Apoptosis in Rat Liver after Perfusion with 3-Amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1)

Abstract

We previously demonstrated that 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) induced apoptosis in primary cultured rat hepatocytes. In this study, we investigated apoptotic biomarkers in rat liver after perfusion with 30 μM Trp-P-1 as preliminary experiments for in vivo study. Induction of c-Myc and p53 protein and the activities of caspase-3, -6, and -8 were detected in Trp-P-1-perfused liver. In addition, Trp-P-1 modulated the DNA binding activity of the apoptosis-related transcription factors, NF-κB and AP-1. These results imply a possibility that Trp-P-1 would induce apoptosis in vivo.

• heterocyclic amine
• Trp-P-1
• apoptosis
• NF-κB
• hepatocytes